Sheep IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Sheep IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivity	Sheep
Host	Rabbit
Immunogen	Sheep IgG whole molecules.
Formulation	0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 containing 10 mg/ml Bovine Serum Albumin (BSA IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative. Label: AP State: Liquid purified Ig fraction. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration	lot specific
Conjugation	AP
Storage Condition	Store the antibody (undiluted) at 2-8°C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.