Human IgA (Secretory component) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Human IgA (Secretory component) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, IP
Recommend Dilution	Can be used as reagent for the direct detection of secretory component in human cells, tissues and body fluids in Immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: 1/20-1/80.
Reactivity	Human
Host	Goat
Immunogen	Purified free Human Secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: FITC State: Lyophilized purified hyperimmune IgG fraction Label: Fluorescein Isothiocyanate isomer 1 (FITC) Absorption emission: 492 nm / 515 nm Molar radio: Fluorescein/IgG ~1.6
Concentration	10 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and purification of the IgG fraction by DEAE-Chromatography.
Conjugation	FITC
Storage Condition	Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

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