Human Monoclonal Antibody [Clone ID: C3_Hu10]

Specifications

Product Data
Clone Name	C3_Hu10
Applications	ELISA, FC, IF, WB
Reactivity	Human, Mouse
Host	Human
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing Balb/c and Trianni mice with human laminin-2 LG5 domain. Humanized version was generated by grafting CDRs of parental mouse antibody C3 onto human framework regions.
Specificity	This antibody recognizes human and mouse laminin-2 LG5 and LG4/5 domain. Laminin is a large extracellular heterotrimeric glycoprotein and a major constituent of the basement membrane. It is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The binding specificity of this antibody for human and mouse laminin-2 LG5 was confirmed using ELISA. This antibody is capable of detecting human and mouse LG-4/5 expressed on HEK293 in a flow cytometry. This antibody can also be used for the identification recombinant human laminin-2 (Hu 211), murine LG-5 (mLG5), human LG-5 (hLG5), and human LG-4/5 (hLG4/LG5) in a western blot. This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. This antibody was used in combination with anti-laminin-2 antibody AS30_Hu6 to generate a duobody called TBTI (US11254737).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Database Link	UniProt ID P24043; Q60675
Synonyms	C3; g-like 4 and 5 domain; humanized C3; LAMA2; Laminin-2 subunit alpha; Laminin-4 subunit alpha; Laminin-12 subunit alpha; Laminin 211; laminin G-like 5 domain; Laminin M chain; Laminin subunit alpha-2; LG-4/5; LG5; Merosin; Merosin heavy chain
Reference Data

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