LPL Rabbit Monoclonal Antibody [Clone ID: 5D2]

Specifications

Product Data
Clone Name	5D2
Applications	ELISA, ICC, IF, Neutralize, WB
Reactivity	Human, Rat, Chicken, Guinea Pig, Bovine, Feline
Modifications	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing mice with bovine LPL.
Specificity	The antibody is specific for lipoprotein lipase (LPL) and it binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The antibody does not cross react with mouse LPL. LPL is a key enzyme in triglyceride metabolism, it catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. LPL was immunoprecipitated from postheparin plasma using this antibody. The antibody was used both to capture LPL and to detect the bound LPL in a sandwich ELISA, indicating the epitope to be present in duplicate. In another sandwich ELISA analysis, the antibody was used to detect the LPL antigen (Peterson et al., 1992, PMID: 1279089). The antibody detected LPL from human, chicken and guinea pig by western blot analysis. The specificity of the original format of the antibody was confirmed by ELISA analysis (Chang et al., 1998; PMID: 9831623). Pretreatment of LPL with the antibody totally suppressed LPL-induced monocyte adhesion to endothelial cells (Mamputu et al., 1997; PMID: 9323582). Further, LDL was immunoneutralized with the antibody, totally suppressing LPL-induced VSMC proliferation (Mamputu et al., 2000; PMID: 11031206). Immunofluorescence was performed on LPL expressed on CHO-K1 cells using this antibody (Voss et al., 2011; PMID: 21518912). The binding affinity of the antibody to a synthetic LPL peptide containing the Trp-rich loop of human, mouse, bovine, rat and chicken, was measured by surface plasmon resonance.The binding affinity of the antibody to the human, bovine and chicken LPL peptides was high (KD of 0.19, 0.78 nM and 0.34 nM, respectively), the binding affinity for the rat LPL peptide was reduced (Kd= 2.99nM), and no binding to the Trp-rich peptide from mouse LPL was detected. The antibody detected LPL by western blot analysis. By immunocytochemistry, the antibody bound avidly to the wildtype human LPL on CHO cells. The crystal structure of the Fab version of the antibody was solved (Luz et al., 2020; PMID: 32690595).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Database Link	UniProt ID P06858
Synonyms	EC 3.1.1.34; LPL; Phospholipase A1
Reference Data

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