GPC3 Rabbit Monoclonal Antibody [Clone ID: 15G1]

Specifications

Product Data
Clone Name	15G1
Applications	ELISA, FC, IHC
Reactivity	Human, Monkey, Rat
Modifications	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK).
Specificity	This antibody binds an epitope between amino acids 420 to 470 of the human glypican 3 (GPC3). This antibody cross reacts with cynomolgus monkey and rat GPC3. Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC). This antibody can bind human glypican 3 expressed on the surface of 293S cells, HepG2 X1 cells and JHH7 cells in a flow cytometric assay. This antibody reacts with human rat and cynomolgus monkey GPC3 in a flow cytometric assay. The binding characterization of this antibody to huGPC3, cynoGPC3, and HepG2 cells was done using ELISA and immunohistochemistry. (US9926377).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Database Link	UniProt ID P51654
Synonyms	DGSX; Glypican-3; GPC-3; GPC3; GTR2-2; Intestinal protein OCI-5; MXR7; OCI5
Reference Data

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