TIGIT Rabbit Monoclonal Antibody [Clone ID: AET2010]

Specifications

Product Data
Clone Name	AET2010
Applications	ELISA, FC
Reactivity	Human
Modifications	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was isolated from a fully synthetic human single chain antibody phage display library by multiple rounds of bio panning against human TIGIT.
Specificity	This antibody binds the human TIGIT (T cell immunoreceptor with Ig and ITIM domains). TIGIT is a type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins. It is an immune receptor present on some T cells and natural killer cells (NK). It binds the poliovirus receptor (PVR) with high affinity which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding characterization of this antibody for TIGIT was done using ELISA. The IgG1 version of this antibody binds human TIGIT with a binding affinity of Kd= 3.74 nM. This antibody is also capable of blocking the binding of TIGIT to its ligand CD155. The binding of this antibody to membrane bound TIGIT was determined using flow cytometry. The anti-tumor activity of this antibody was tested against NK-92MI cells in vitro. It was also reported that AET 2010 exhibits enhanced anti-tumor activity in the CDX mouse model of NK-92MI cells (CN112679610).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Database Link	UniProt ID Q495A1
Synonyms	T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; WUCAM
Reference Data

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