Rabbit Polyclonal Antibody
CNY 7,519.00
货期*
4周
规格
经常一起买 (1)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
CNY 300.00
CNY 1,430.00
Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommend Dilution | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:80,000 |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | The immunogen is full length GST isolated from Schistosoma japonicum. |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
Concentration | 1.0 mg/mL - lot specific |
Conjugation | Biotin |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Background | GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. |
Synonyms | rabbit anti-GST antibody biotin conjugation, biotin conjugated rabbit anti-GST antibody, Glutathione-S-Transferase |
Note | Anti-GST Biotin Conjugated Antibody has been tested by ELISA and is suitable for ELISA, western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency. |
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