SMAD2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1,000 - 1:3,000 ELISA: 1:10,000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	SMAD2 pS465 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.
Specificity	Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human SMAD2. Reactivity with non-phosphorylated human Smad2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.94 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	SMAD family member 2
Database Link	Entrez Gene 4087 Human UniProt ID Q15796
Background	Smad2 is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). Smad2 (Mothers against decapentaplegic homolog 2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Anti-SMAD2 pS465 pS467 antibody is ideal for researchers interested in Cancer, Immunology and Nuclear Signaling research.
Synonyms	rabbit anti-SMAD2 pS465pS467 antibody, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2 antibody, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1
Note	Anti-SMAD2 pS465 pS467 affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 52 kDa in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.