Huntingtin (HTT) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1ug/ml IHC: User Optimized ELISA: 1: 10,000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-Huntington pS421 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Huntington disease protein.
Specificity	Anti-Huntingtin pS421 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of the S421 residue. A BLAST analysis was used to suggest cross-reactivity with human based on 100% sequence homology. Cross-reactivity with Huntington pS421 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Gene Name	huntingtin
Database Link	Entrez Gene 3064 Human UniProt ID P42858
Background	Huntington's disease (HD) is an autosomal dominant neurological disorder caused by a polyglutamine (polyQ) repeat expansion in the huntingtin (HTT) protein. The disease is characterized by neurodegeneration and formation of neuronal intracellular inclusions primarily in the striatum and cortex, leading to personality changes, motor impairment, and dementia. The Huntington’s disease protein is ~350kD in size and is localized in the brain. Anti-Huntington antibody is ideal for investigators interested in Neuroscience research.
Synonyms	rabbit anti-Huntington pS421 antibody, rabbit anti-Huntingtin pS421 antibody, HD protein, HTT, Huntington Disease Protein, Huntington disease, IT15, LOMARS
Note	Anti-HTT antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350kDa corresponding to the appropriate cell lysate or extract.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.