Nfe2l2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1.0 ug/ml ELISA: 5 ug/ml
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-NRF-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding near the c-term region.
Specificity	This affinity-purified antibody is directed against mouse Nrf2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Nrf2 proteins from human, mouse, rat and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	nuclear factor, erythroid derived 2, like 2
Database Link	Entrez Gene 18024 Mouse UniProt ID Q60795
Background	Anti-Nrf-2 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Nrf2 links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Nrf2 is localized in the cytoplasm and nucleus and ubiquitously expressed with the highest concentrations in the kidney, muscle, lung, heart, liver, and brain. Anti-Nrf2 antibody is useful for researchers interested in cellular growth, transcription factor, and cancer research.
Synonyms	rabbit anti-Nrf2 antibody, Nrf 2, Nrf-2 Antibody, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, Nfe2l2, HEBP1
Note	This affinity purified Nrf2 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66.9 kDa in size corresponding to Nrf-2 proteins by western blotting in the appropriate cell lysate or extract.
Reference Data

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