Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IHC, WB
Recommend Dilution	WB: 1:500 -1:3,000 IHC: 1:250 -1:1,500 FC: User Optimized ELISA: 1:5,000 -1:30,000
Host	Goat
Clonality	Polyclonal
Immunogen	Fluorescein conjugated to Goat IgG
Specificity	Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background	Anti-Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).
Synonyms	goat Anti-FITC antibody, goat anti-fluorescein antibody
Note	Anti-Fluorescein Antibody has been tested by ELISA and western blot and is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.