Transferrin (TF) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	WB: User Optimized IF: User Optimized ELISA: 1:10,000 - 1:20,000 FLISA: User Optimized
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Transferrin (Human Serum)
Specificity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Transferrin (Human Serum).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
Concentration	10.0 mg/mL - lot specific
Conjugation	FITC
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Gene Name	transferrin
Database Link	Entrez Gene 7018 Human UniProt ID P02787
Background	Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.
Synonyms	rabbit anti-Transferrin Antibody Fluorescein Conjugated, Apotransferrin antibody, Beta 1 metal binding globulin antibody, DKFZp781D0156 antibody, PRO1400 antibody, PRO1557 antibody, PRO2086 antibody, Serotransferrin precursor antibody, Siderophilin antibody, TF antibody
Note	Anti-Human transferrin Fluorescein has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reference Data

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