PRXC, HRPC Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 - 1:3,000 ELISA: 1:2,000 - 1:10,000
Host	Sheep
Clonality	Polyclonal
Immunogen	Peroxidase [Horseradish]
Specificity	Anti-PEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Peroxidase [Horseradish].  Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Background	Peroxidase described as the removal of hydrogen peroxide, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, a response to environmental stresses such as wounding, a and pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Synonyms	sheep anti-Peroxidase Antibody, sheep anti-HRP antibody, horseradish antibody, sheep anti Peroxidase, sheep anti HRP
Note	Anti-Peroxidase has been tested by western blot and is suitable for dot blot, ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Reference Data

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