DNASE1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Deoxyribonuclease I [Bovine Pancreas]
Specificity	Anti-Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas]. Cross reactivities against Deoxyribonuclease I from other sources may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P00639
Background	Anti-Deoxyribonuclease I antibody is primarily involved in cell death by apoptosis, though it performs other various functions. Deoxyribonuclease I binds specifically to G-actin and blocks actin polymerization. Belonging to the DNase I family, Anti-Deoxyribonuclease antibody is ideal for researchers interested in Metabolism, Epigenetics and Nuclear Signaling, and Cell Biology.
Synonyms	rabbit anti-Deoxyribonuclease I Antibody biotin Conjugation, biotin conjugated rabbit anti-Deoxyribonuclease I Antibody, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I
Note	Anti-Deoxyribonuclease I Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Deoxyribonuclease I in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Reference Data

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