DLD Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000
Reactivity	Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Lipoamide Dehydrogenase [Porcine Heart]
Specificity	Anti-Lipoamide dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].  Cross reactivity against Lipoamide Dehydrogenase from other sources may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Database Link	UniProt ID P09623
Background	Anti-Lipoamide dehydrogenase recognizes the protein lipoamide dehydrogenase. Lipoamide dehydrogenase catalyzes the conversion of lipoamide to dihydrolipoamide with the concomitant oxidation of NADH to NAD+. Lipoamide dehydrogenase is known to play a role in alpha-ketoacid dehydrogenase complexes as well glycine cleavage system. Defects in lipoamide dehydrogenase result in congenital infantile acidosis and maple syrup urine disease. Anti-Lipoamide dehydrogenase is suitable for investigators interested in metabolism, neurology, and cancer.
Synonyms	rabbit anti-Lipoamide Dehydrogenase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase
Note	Anti-Lipoamide dehydrogenase Biotin Conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Lipoamide Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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