CA2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:200 - 1:1,000 ELISA: 1:2,000 - 1:10,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Carbonic Anhydrase II [Bovine Erythrocytes]
Specificity	Anti-Carbonic Anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].  Cross reactivity against Carbonic Anhydrase II from other sources may occur but has not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P00921
Background	Anti-Carbonic Anhydrase II antibody is necessary for bone resorption and osteoclast differentiation and is involved in the reversible hydration of carbon dioxide. Belonging to the alpha-carbonic anhydrase family, Anti-Carbonic Anhydrase II antibody is ideal for investigators involved in the areas of Cardiovascular and Cell Biology research.
Synonyms	rabbit anti-Carbonic Anhydrase II Antibody biotin Conjugation, biotin conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II
Note	Anti-Carbonic Anhydrase II Biotin Conjugated Antibody has been tested against 1.0 ug of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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