CAT Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500- 1:2,500 ELISA: 1:10,000 - 1:40,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Catalase [Bovine Liver]
Specificity	Anti-Catalase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine Liver].  Cross reactivity against Catalase from other sources may occur but has not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Database Link	UniProt ID P00432
Background	Anti-Catalase antibody serves to protect cells of aerobically respiring organisms from the negative effects of hydrogen peroxide. Additionally Catalase promotes cell growth including T-cells, B-cells, myeloid leukemia, melanoma, mastocytoma, and normal and transformed fibroblast cells. Belonging to the catalase family, Anti-Catalase antibody is ideal for researchers invested in Metabolism, Cancer , Cardiovascular , Signal Transduction, and Tags & Cell Marker research.
Synonyms	rabbit anti-Catalase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody
Note	Anti-Catalase Biotin Conjugated Antibody has been tested by ELISA and western blot. This antibody is assayed against 1.0 ug of Catalase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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