BMY1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 - 1:3,000 ELISA: 1:5,000 - 1:25,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Beta Amylase [Sweet Potato]
Specificity	Anti-Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato]. Cross reactivity against b-Amylase from other sources may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID P10537
Background	Anti-Beta Amylase antibody works through the hydrolysis of 1,4 alpha-D-glucosidic linkages in polysaccharides to remove successive maltose units from the non-reducing ends of the chains. Anti-Beta Amylase is often used in the structural analysis of starch and glycogen and is found in the cytoplasmic region. Anti-Beta Amylase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cell Biology research.
Synonyms	rabbit anti-Beta Amylase Antibody biotin Conjugation, biotin conjugated rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody
Note	Anti-Beta Amylase Biotin Conjugated Antibody has been tested by dot blot and western blot. This product is assayed against 1.0 ug of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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