GLUD1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Glutamate Dehydrogenase [Bovine Liver]
Specificity	Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID P00366
Background	Glutamate Dehydrogenase, mitochondrial, converts L-glutamate into alpha-ketoglutarate. It plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, which is an important intermediate in the tricarboxylic acid cycle. It may be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. It is subject to allosteric regulation. It is activated by ADP, inhibited by GTP and ATP, and ADP can occupy the NADH binding site and activate the enzyme.
Synonyms	rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1
Note	Glutamate Dehydrogenase antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Glutamate Dehydrogenase [Bovine Liver] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product.
Reference Data

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