BMY1 Rabbit Polyclonal Antibody

CAT#: TA396941S

Beta Amylase Antibody



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CNY 2,119.00


货期*
4周

规格
    • 25 ul

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution WB: 1:500 - 1:3,000
ELISA: 1:5,000 - 1:25,000
Host Rabbit
Clonality Polyclonal
Immunogen Beta Amylase [Sweet Potato]
Specificity Anti-BETA AMYLASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b Amylase [Sweet Potato].  Cross reactivity against b Amylase from other sources may occur but has not been specifically determined.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.0 mg/ml - lot specific
Conjugation Unconjugated
Storage Condition Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background Beta Amylase has catalytic activity of hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
Synonyms rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody
Note Beta Amylase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of b Amylase [Sweet Potato] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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