AGAP8 (AGAP4) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1000 ELISA: 1:10,000-1:20,000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-AGAP4 antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant His-tagged AGAP4 protein.
Specificity	Anti-AGAP4 antibody was purified from monospecific antiserum by protein A affinity purification and the His-tag was removed by cross adsorption. An immunoblot assay detects overexpressed recombinant AGAP4.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.04mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Gene Name	ArfGAP with GTPase domain, ankyrin repeat and PH domain 4
Database Link	Entrez Gene 119016 Human UniProt ID Q96P64
Background	This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). The AGAPs are the largest subgroup of the ArfGAP family with 11 genes in humans. AGAP4 (also known as Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 4) is a GTPase activating protein. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. AGAP4 and other AGAP proteins likely function in the secretory pathway, which could explain a link with the invasive behavior of cancer cells. Reliable antibodies would be invaluable for studies of the physiologic function of the proteins and how is may be linked to cancer cell invasive behavior. Anti-AGAP4 Antibody is useful for researchers interested in Developmental Biology, apoptosis, Cancer, Immunology, and Nuclear Signaling research.
Synonyms	rabbit anti-AGAP4 antibody, AGAP-4, AGAP 4, CTGLF1, MRIP2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 4, Centaurin-gamma-like family member 1
Note	Anti-AGAP4 antibody has been tested for use in western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 73kDa in size corresponding to AGAP4 by western blotting in the appropriate cell lysate or extract.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.