ErpA8 Rabbit Polyclonal Antibody

CAT#: TA396876S

ErpN/OspE Antibody

Size: 100 ug 25 ul



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CNY 2,119.00


货期*
4周

规格
    • 25 ul

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution WB: 1:1,000
ELISA: >1:5,000
Reactivity Borrelia burgdorferi
Host Rabbit
Clonality Polyclonal
Immunogen MBP-fusion protein corresponding to Borrelia burgdorferi ErpN/OspE protein.
Specificity This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. It is directed against, and shows specific reactivity for, Borrelia burgdorferi OspE protein. Reactivity with ErpN/OspE protein from other sources has not been determined.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.1 mg/mL - lot specific
Conjugation Unconjugated
Storage Condition Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background This product is antibody made against ErpN (OspE/F-Related Protein N), from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. Erp proteins from Borrelia burgdorferi are postulated to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, indicative of Erp synthesis during the initial stages of vertebrate infection. It is postulated that surface-exposed Erp proteins could facilitate interactions with host tissues during the establishment of vertebrate infection.
Synonyms Rabbit anti-ErpN Antibody, rabbit anti-OspE Antibody, rabbit anti-ErpN/OspE Antibody, ErpA, outer surface protein E, Borrelia burgdorferi OspE, ospE/F-Related Protein N, ErpA8, BB_L39, cp32-8
Note Anti-ErpN/OspE antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at 17.1 kDa in size corresponding to ErpN/OspE by Western blotting in the appropriate cell lysate or extract.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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