MAD1 (MAD1L1) Mouse Monoclonal Antibody [Clone ID: 9B10]

Specifications

Product Data
Clone Name	9B10
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:200 - 1:1,000 ELISA: 1:5,000 - 1:20,000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD1L1 protein.
Specificity	This Protein A purified antibody is directed against human MAD1L1 protein. The product was purified from tissue culture supernatant by chromatography.  This antibody has only been tested on human cells.  Reactivity against homologues from other sources is not known.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Gene Name	MAD1 mitotic arrest deficient like 1
Database Link	Entrez Gene 8379 Human UniProt ID Q9Y6D9
Background	MAD1L1 (also called mitotic spindle assembly checkpoint protein, MAD1A, MAD1-like 1 and HsMAD1) is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD1L1 has a role in the correct positioning of the septum and is required for anchoring MAD2L1 to the nuclear periphery.   MAD1L1 forms a homodimer and also heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20.  MAD1L1 is also able to interact with the BUB1/BUB3 complex and the viral Tax protein.   MAD1L1 is a nuclear protein that is seen to move from the beginning to the end of mitosis from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody.  Multiple isoforms may exist for this protein (MAD1L1 and MAD1L2).  MAD1L1 is induced by TP53 and is phosphorylated by BUB1. MAD1L1 is hyperphosphorylated in late S through M phases or after mitotic spindle damage.  Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
Synonyms	mouse anti-MAD1L1 antibody, Mitotic arrest deficient 1 antibody, Mitotic checkpoint MAD1 protein homolog antibody, Mitotic spindle assembly checkpoint protein MAD1 antibody, PIG9 antibody, Tax binding protein 181 antibody
Note	This protein A purified antibody is suitable for use in flow cytometry, immunoprecipitation, immunofluorescence and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 83 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Reference Data

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