ACHE Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:2,500 ELISA: 1:2,000 - 1:10,000
Host	Goat
Clonality	Polyclonal
Immunogen	Acetyl Cholinesterase [Bovine Erythrocytes]
Specificity	Acetyl Cholinesterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].  Cross reactivity against Acetyl Cholinesterase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P23795
Background	Acetyl Cholinesterase is abundantly expressed in erythrocytes and is part of the type-B carboxylesterase/lipase family. Anti-Acetyl Cholinesterase cancels signal transduction at the neuromuscular junction via hydrolysis of acetylcholine as it is released into the synaptic cleft by the motor neurons. Anti-Acetyl Cholinesterase antibody is ideal for investigators involved in Neuroscience and Neurotransmitter research.
Synonyms	goat anti-Acetyl Cholinesterase Antibody, biotin conjugated goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE
Note	Anti-Acetyl Cholinesterase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 is suggested for this product.
Reference Data

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