MYO6 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1000 ELISA: 1:10,000
Reactivity	Human, Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with a full length myosin-6 construct expressed in SF9 insect cells corresponding to porcine myosyn-6 protein.
Specificity	Anti-Myosin-6 is directed against the myosin-6 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with 94% human Myosin VI. Cross-reactivity with myosin6 from other sources have not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	25 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID Q29122
Background	Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism.
Synonyms	rabbit anti-Myosin-6 Antibody, MyosinVI, Unconventional myosin 6
Note	Myosin-6 antibody has been tested by western blot. For western blots expect a band of approximately 150 kDa in size corresponding to full length and ~85kDa and ~60kDa for truncated myosin-6 protein. Specific conditions for reactivity should be optimized by the end user. This antibody is suitable for use in ELISA.
Reference Data

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