C3AR1 Polyclonal Antibody [Clone ID: HC3aRZ8]

Specifications

Product Data
Clone Name	HC3aRZ8
Applications	FC
Recommend Dilution	Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Reactivity	Human
Clonality	Polyclonal
Immunogen	Human C3aR transfectants
Specificity	The mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin.
Formulation	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Format: PE Label: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Concentration	lot specific
Purification	*Abdelbaset-Ismail A, Borkowska-Rzeszotek S, Kubis E, Bujko K, Brzeźniakiewicz-Janus K, Bolkun L, Kloczko J, Moniuszko M, Basak GW, Wiktor-Jedrzejczak W, Ratajczak MZ: Activation of the complement cascade enhances motility of leukemic cells by downregulating expression of HO-1. Leukemia 2017 Feb;31(2):446-458., URL: https://pubmed.ncbi.nlm.nih.gov/27451975/,*Larsen LF, Juel-Berg N, Hansen A, Hansen KS, Mills EN, van Ree R, Rådinger M, Poulsen LK, Jensen BM: No difference in human mast cells derived from peanut allergic versus non-allergic subjects. Immun Inflamm Dis. 2018 Dec;6(4):416-427, URL: https://pubmed.ncbi.nlm.nih.gov/29992767/,*Reis ES, Chen H, Sfyroera G, Monk PN, Köhl J, Ricklin D, Lambris JD: C5a receptor-dependent cell activation by physiological concentrations of desarginated C5a: insights from a novel label-free cellular assay. J Immunol. 2012 Nov 15;189(10):4797-805. , URL: https://pubmed.ncbi.nlm.nih.gov/23041570/,*Soruri A, Kiafard Z, Dettmer C, Riggert J, Köhl J, Zwirner J: IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo. J Immunol. 2003 Mar 15;170(6):3306-14., URL: https://pubmed.ncbi.nlm.nih.gov/12626590/,*Kiafard Z, Tschernig T, Schweyer S, Bley A, Neumann D, Zwirner J: Use of monoclonal antibodies to assess expression of anaphylatoxin receptors in tubular epithelial cells of human, murine and rat kidneys. Immunobiology 2007;212(2):129-39., URL: https://pubmed.ncbi.nlm.nih.gov/17336833/
Conjugation	Unconjugated
Storage Condition	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Gene Name	complement component 3a receptor 1
Database Link	Entrez Gene 719 Human UniProt ID Q16581
Synonyms	AZ3B, HNFAG09
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.