SHC (SHC1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:500~1:1000 IHC: 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human Shc around the phosphorylation site of Tyrosine 349.
Specificity	p-Shc (Y349) polyclonal antibody detects endogenous levels of Shc protein only when phosphorylated at Tyr349.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 46, 52, 66 kDa
Gene Name	SHC adaptor protein 1
Database Link	Entrez Gene 6464 Human UniProt ID P29353
Background	Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.
Synonyms	SH2 domain protein C1; SHC; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; SHC1; SHCA; Src homology 2 domain-containing-transforming protein C1
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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