IKK gamma (IKBKG) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:1000~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human IKKγ around the phosphorylation site of Serine 31.
Specificity	IKKγ (Phospho-S31) polyclonal antibody detects endogenous levels of IKKγ protein only when phosphorylated at Ser31.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 54 kDa
Gene Name	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Database Link	Entrez Gene 8517 Human UniProt ID Q9Y6K9
Background	Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates I˚Bα on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.
Synonyms	FIP-3; FIP3, NEMO; I-kappa-B kinase subunit gamma; IKBKG; IkB kinase-associated protein 1; IkB kinase subunit gamma; IKK-gamma; IKKAP1; IKKG; Inhibitor of nuclear factor kappa-B kinase subunit gamma; NEMO; NF-kappa-B essential modifier; NF-kappa-B essential modulator
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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