VIM Mouse Antibody [Clone ID: V9]

Specifications

Product Data
Clone Name	V9
Applications	ICC, WB
Recommend Dilution	WB: 1:200 ICC: 1:100
Reactivity	Human, Rat, Mouse, Chicken
Host	Mouse
Immunogen	Clone (V9) was generated from full length vimentin purified from porcine eye lens. The antibody reacts with human, pig, chicken, rat, and mouse vimentins.
Specificity	The antibody detects a 58 kDa* protein corresponding to the molecular mass of vimentin on SDS-PAGE immunoblots of human fibroblasts (HS-68). In immunocytochemistry, the antibody detects intermediate filaments in rat smooth muscle cells (A7r5).
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	58
Database Link	UniProt ID P08670
Background	Intermediate filaments (IF) are important components of the cytoskeleton and nuclear envelope. IF protein family members are encoded by 70 genes that have diverse expression patterns during development and tissue-specific functions. For example, the keratins form IFs in epithelial cells, while vimentin forms filaments in mesenchymal, endothelial, and hematopoietic cells. Neurofilament triplet proteins and internexins form IFs in neurons, while desmin, synemin and syncoilin form the extra-sarcomeric cytoskeleton of myoblasts. The nuclear IF system consists of lamin family proteins organized in a meshwork-like lamina around the nucleus. Vimentin is a 58 kDa IF protein that forms 10 nm filaments that are important for mechanical stability, motility, and adhesion in various cell types. Vimentin activity is regulated by proteolysis and phosphorylation. Rho-kinases can phosphorylate vimentin leading to destabilization of intermediate filaments, while Akt phosphorylation of Ser-39 in vimentin reduces proteolysis of vimentin and promotes cancer cell motility and invasion.
Note	Protein G purified tissue culture supernatant.
Reference Data

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