STAT1 Mouse Antibody [Clone ID: M249]

Specifications

Product Data
Clone Name	M249
Applications	ICC, IP, WB
Recommend Dilution	WB: 1:1000 ICC: 1:100
Reactivity	Human, Rat, Mouse
Host	Mouse
Immunogen	Clone (M249) was generated from a recombinant protein from human Stat1. This sequence has high homology to the conserved region in rat and mouse stat1 a and b.
Specificity	The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human Jurkat and A431 cells.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	84/91
Database Link	UniProt ID P42224
Background	The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat1 is expressed as two variants of 84 and 91 kDa. Stat1 proteins contain SH2 and SH3 domains, and are components of the interferon-stimulated gene factor 3 (ISGF3) complex. This complex is the primary transcription activator induced by the binding of interferon to its receptors. In response to activation by various cytokines and growth factors, stat1 subunits become phosphorylated at tyrosine 701. This leads to translocation of stat1 to the nucleus, resulting in formation of an active ISGF3 complex. Active ISGF3 modulates the transcription of the interferon-stimulated genes. Thus, phosphorylation of Tyr-701 is critical for gene expression mediated by various cytokines and growth factors.
Note	Protein G purified tissue culture supernatant.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.