(pSer/Thr) Rabbit Antibody
CAT#: TA389186
Anti-Anti-Phosphoserine/threonine Antibody
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CNY 4,069.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ICC, IP, WB |
Recommend Dilution | WB: 1:1000 ICC: 1:50 |
Reactivity | Human, Rat, Mouse |
Host | Rabbit |
Immunogen | Anti-Phosphoserine/threonine was generated from a panel of phosphoserine and phosphothreonine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved in many species. |
Specificity | This antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using a mix of phosphoserine and phosphothreonine peptides (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA. |
Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Concentration | lot specific |
Purification | Antigen Affinity Purified |
Conjugation | Unconjugated |
Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Background | Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity. |
Note | Antigen affinity purified rabbit serum. |
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