ATP1B3 Mouse Antibody [Clone ID: M016]

Specifications

Product Data
Clone Name	M016
Applications	ICC, IP, WB
Recommend Dilution	WB: 1:1000 ICC: 1:50
Reactivity	Human
Host	Mouse
Immunogen	Clone (M016) was generated from a proprietary antigen related to the native human Na+/K+ ATPase β3 subunit expressed in A431 epidermoid carcinoma cell line.
Specificity	Clone M016 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase β3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects Na+/K+ ATPase β3 protein.The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the β3 subunit on live cells.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	40
Database Link	UniProt ID P54709
Background	The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic α subunit and a membrane-spanning auxiliary β subunit. In humans, the Na+/K+ ATPase is a binary complex of an α subunit that has four isoforms (α1-α4) and a β-subunit that has three isoforms (β1, β2, β3). Na+/K+ ATPase subunit expression has been shown to be upregulated in cancers, and inhibition of Na+/K+ ATPase activity has anti-cancer effects. The β3 subunit of Na+/K+ ATPase has increased expression in human gastric cancer tissues and cell lines, and its increased expression level predicts poor patient outcome. β3 subunit knockdown significantly inhibited cell proliferation, colony-formation ability, migration, and invasion in human gastric carcinoma cell lines.
Note	Protein G purified tissue culture supernatant.
Reference Data

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