Phospho-MAPK8 Mouse Antibody [Clone ID: M268]

Specifications

Product Data
Clone Name	M268
Applications	ICC, WB
Recommend Dilution	WB: 1:1000 ICC: 1:100
Reactivity	Human, Rat, Mouse
Host	Mouse
Immunogen	Clone M268 was generated from a dual phosphorylated peptide corresponding to amino acid residues surrounding Thr-183 and Tyr-185 in human JNK1. This sequence has high homology to the conserved site in rat and mouse JNK1 and JNK2.
Specificity	This antibody detects 46 and 54 kDa* proteins corresponding to the apparent molecular mass of JNK1 and JNK2 on SDS-PAGE immunoblots of human A431 and HeLa cells, as well as rat PC12 cells treated with calyculin A.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	46/54
Database Link	UniProt ID P45983
Background	The stress-activated protein kinases (SAPK) or Jun-amino-terminal kinases (JNK) are potently activated by stressors such as UV and gamma radiation. Similar to other MAP Kinases, the core signaling unit is composed of a MAPKKK, usually MEKK1-4 or a mixed lineage kinase (MLK), which phosphorylate and activate MKK4-7, leading to dual phosphorylation and activation of JNK kinases. Rho-GTPases (Rac1 and cdc42) can stimulate MEKKs and MLKs, while MKKs can be activated by a GTPase-independent pathway that involves the germinal center kinase family. There are three JNK genes (JNK1, 2, 3) with further diversification resulting from alternative splicing. Active JNK dimers can translocate to the nucleus to regulate transcription through phosphorylation of c-Jun, ATF-2 and other transcription factors.
Note	Protein G purified tissue culture supernatant.
Reference Data

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