ABL1 Mouse Antibody [Clone ID: M209]

Specifications

Product Data
Clone Name	M209
Applications	WB
Recommend Dilution	WB: 1:1000
Reactivity	Human, Rat, Mouse
Host	Mouse
Immunogen	Clone (M209) was generated from a recombinant protein corresponding to the C-terminal region of human c-Abl.
Specificity	This antibody detects a 145 kDa* protein corresponding to c-Abl on SDS-PAGE immunoblots of human K-562 and HL-60 cells, and mouse ANN-1 cells.
Formulation	PBS + 0.2% gelatin and 0.09% NaN3.
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Predicted Protein Size	145
Database Link	UniProt ID P00519
Background	The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is widely expressed and is distributed in both the nucleus and the cytoplasm of cells. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion, and stress response. A variety of stimuli activate c-Abl kinase including integrin activation, PDGF stimulation, and binding to proteins, such as c-Jun. Tyrosine phosphorylation is important for the regulation of c-Abl kinase activity. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains. Phosphorylation of Tyr-245 is involved in activation of c-Abl kinase activity. Tyrosine 412 is located in the kinase activation loop of c-Abl, and phosphorylation of this residue is required for kinase activity. Thus, phosphorylation of Tyr-245 and Tyr-412 may be critical for activation of c-Abl in a variety of cell signaling pathways.
Note	Protein G purified tissue culture supernatant.
Reference Data

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