Chrnb4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000 WB Brain: 1:1000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein from the cytoplasmic loop of the β4 subunit of mouse nAChR.
Specificity	Specific for endogenous levels of the ~52 kDa nAChRβ4 protein.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Concentration	lot specific
Purification	Antigen Affinity Purified from Pooled Serum
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	52
Gene Name	cholinergic receptor, nicotinic, beta polypeptide 4
Database Link	Entrez Gene 108015 Mouse UniProt ID Q8R493
Background	Nicotinic acetylcholine receptors (nAChRs) are ionotropic, cholinergic receptors that are divided into 2 types; muscle type and neuronal type. Neuronal nAChRs are pentameric ion channels consisting of 5 identical (homopentamers) or different (heteropentamers) subunits. Heteropentameric neuronal nAChRs mediate fast synaptic transmission in the autonomic nervous system. The predominant hetero-oligomeric nAChR in the CNS contain the subunits α4β2, whereas α3β4 prevail in the PNS. However, the expression of these subunits varies not only by region but also during development (Scholze et al 2011). In the brain, β2-containing receptors greatly outnumber receptors that contain β4 (McGehee & Role, 1995; Albuquerque, et al., 2009), and in most brain regions, targeted deletion of the β2 subunit virtually abolishes [3H]-epibatidine binding and receptor autoradiography (Zoli, et al., 1998) due to the absence of a β subunit required to form functional nAChRs (Champtiaux & Changeux, 2004).
Synonyms	CHRNB4
Note	Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein antigen was coupled.
Reference Data

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