Phospho-Slc12a3 (pThr53) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:1000-1:6000 WB Brain: 1:1000-1:6000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH).
Specificity	Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Concentration	lot specific
Purification	Antigen Affinity Purified from Pooled Serum
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	160
Gene Name	solute carrier family 12, member 3
Database Link	Entrez Gene 20497 Mouse UniProt ID P55017
Background	The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).
Synonyms	FLJ96318; NCCT; TSC
Note	Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reference Data

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