Phospho-Dennd3 (pSer554) Rabbit Polyclonal Antibody
CNY 5,325.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | WB |
Recommend Dilution | WB: 1:500 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH). |
Specificity | Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Ser554. The immunolabeling is completely eliminated by treatment with λ-phosphatase. |
Formulation | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
Concentration | lot specific |
Purification | Antigen Affinity Purified from Pooled Serum |
Conjugation | Unconjugated |
Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Predicted Protein Size | 142 |
Gene Name | DENN domain containing 3 |
Database Link | |
Background | The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (Marat, A.L., et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (Xu and McPherson, 2015; Matsui et al., 2011). ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (Xu and McPherson, 2015). |
Synonyms | KIAA0870 |
Note | Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phospho peptide affinity columns. |
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