CDw75 (ST6GAL1) Mouse Monoclonal Antibody [Clone ID: LN1]

Specifications

Product Data
Clone Name	LN1
Applications	IHC, WB
Recommend Dilution	WB: 1:100
Host	Mouse
Clonality	Monoclonal
Immunogen	Nuclei isolated from pokeweed mitogen-stimulated human peripheral blood lymphocytes.
Specificity	Specific for endogenous levels of the ~35 kDa CD75 protein.
Formulation	PBS
Concentration	lot specific
Purification	Protein L purified
Conjugation	Unconjugated
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Predicted Protein Size	35
Gene Name	ST6 beta-galactoside alpha-2,6-sialyltransferase 1
Database Link	UniProt ID P15907
Background	CD75, also known as β-galactoside α2,6-sialyltransferase, is a specific carbohydrate antigen that is predominantly expressed on B cell lymphocytes. β-galactoside α2,6-sialyltransferase is an enzyme that catalyzes the addition of sialic acid to growing carbohydrate chains of glycoproteins (Guy, K., et al, 1991). CD75 is a major component of the lymphoid cell surface that serves as a ligand for cell adhesion molecules (Paulson, J.C., et al, 1989). The LN-1 clone reacts positively with red blood cell precursors of the bone marrow, ciliated epithelial cells of the bronchus, distal tubular cells of the kidney, and ductal cells from several organs including the breast and prostate (Epstein, A.L., et al, 1984). CD75 has been shown to be superior to other B cell markers in detecting lymphocyte predominant cells through their cytoplasmic and membraneous staining, especially in typical nodular lymphocyte-predominant Hodgkin lymphoma patterns (Carbone, A., et al, 2014).
Synonyms	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2; MGC48859; OTTHUMP00000210002; SIAT1; ST6GalI; ST6N
Note	Protein L purified culture supernatant.
Reference Data

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