Aqp4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000 WB Brain: 1:1000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat AQP4, conjugated to keyhole limpet hemocyanin (KLH).
Specificity	Specific for endogenous levels of the ~35 kDa aquaporin 4 protein.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Concentration	lot specific
Purification	Antigen Affinity Purified from Pooled Serum
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	35
Gene Name	aquaporin 4
Database Link	Entrez Gene 25293 Rat UniProt ID P47863
Background	Aquaporin-4 (AQP4), a bidirectional water channel protein, is the most expressed aquaporin within the central nervous system. AQP4 is predominantly expressed by astrocytes and ependymal cells within the blood-brain-barrier and ependymal-cerebrospinal fluid barriers (Verkman, et al 2011). AQP4 plays a role in synaptic plasticity (Skucas et al, 2011), astrocyte mitigation (Saadoun et al, 2005), and K+ homeostasis (Binder et al, 2006). Due to the significant role AQP4 plays in cognition, it has been reported to be dysregulated in several neurological disorders. Alzheimer’s patients have amyloid deposits in the walls of the vasculature known as CAA which causes AQP4 mis-location (Wilcock et al, 2009). Patients with Parkinson’s disease have low levels of AQP4 expression which leads to reduced inflammatory response (Chi et al,2011). Reduced levels of AQP4 in traumatic brain injury affects both the acute stage, decreasing the ability to remove excess water from the brain, and in the later stage, by preventing cellular damage and swelling (Zhang et al, 2015).
Synonyms	AQP-4; aquaporin-4; HMIWC2; MGC22454; MIWC; WCH4
Note	Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column prepared with the C-terminal peptide used as antigen.
Reference Data

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