IL12A , IL12B Goat Polyclonal Antibody
CNY 7,481.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Sandwich ELISA: To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-12 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. Western Blot To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL12. Anti-Human IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix. |
Concentration | lot specific |
Purification | IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix |
Conjugation | Unconjugated |
Storage Condition | Store at -20°C as received. |
Background | IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues). |
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