CCL11 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Eotaxin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEotaxin. Western Blot To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEotaxin. Anti-Human Eotaxin specific antibody was purified by affinity chromatography and then biotinylated.
Concentration	lot specific
Purification	Eotaxin specific antibody was purified by affinity chromatography and then biotinylated
Conjugation	Biotin
Storage Condition	Store at -20°C as received.
Database Link	UniProt ID P51671
Background	Eotaxin is a CC chemokine that signals through the CCR3 receptor. It is produced by IFN-gamma-stimulated endothelial cells and TNF-activated monocytes. Eotaxin selectively chemoattracts eosinophils and, along with Eotaxin-2 and Eotaxin-3, plays a key role in the regulation of eosinophil recruitment in the asthmatic lung and in allergic reactions. Recombinant Human Eotaxin is an 8.3 kDa protein containing 74 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Reference Data

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