IL12A , IL12B Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml Recombinant Human IL-12 when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Human IL-12 as the detection antibody at a concentration of at least 0.25-0.5 μg/ml. Western Blot To detect Human IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-12 is 0.50-1.0 ng/lane, under non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Produced in BALB/c x ICR F1 mice immunized with highly pure Recombinant Human IL-12. Anti-Human IL- 12-specific antibody was purified from ascites fluid by Protein A affinity chromatography.
Concentration	lot specific
Purification	IL- 12-specific antibody was purified from ascites fluid by Protein A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Background	IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
Reference Data

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