CD89 (FCAR) Rabbit Monoclonal Antibody [Clone ID: 14A8 (14.1)]

Specifications

Product Data
Clone Name	14A8 (14.1)
Applications	ELISA, FC, IP
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing HCO12 trangene containing mice with recombinant soluble CD89 antigen.
Specificity	This antibody binds CD89 in the extracellular domain 2, outside the IgA-binding site. This antibody binds human CD89 with a dissociation constant of Kd= 6.8 X10-10M.The binding of this antibody to human CD89 was confirmed with ELISA. This antibody was also capable of recognizing human CD89 expressing cells like human PMNL cells by flow cytometry. This antibody is not capable of blocking binding of IgA to CD89. This antibody can used to immunoprecipitate soluble CD89 (US7192582). A bispecific antibody construct incorporating the variable regions of the anti-gp41 antibody F240 and the anti-CD89 (immunoglobulin A [IgA] receptor) antibody 14A8 was reported to promote destruction of HIV type 1 (HIV-1) by neutrophils (PMID: 18272577). A Fab-like bispecific antibody molecule or Bi-Fab antibody generated from F240scFv and 14A8scFv showed significant antiboy dependent cell-mediated viral inhibition (ADCVI) activity for multiple clade B and clade C isolates by arming the neutrophils to inhibit HIV infection (PMID: 27419146).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	Fc fragment of IgA receptor
Database Link	Entrez Gene 2204 Human UniProt ID P24071
Synonyms	CD89
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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