CD163 Mouse Monoclonal Antibody [Clone ID: 2A10 (2A10/11)]

Specifications

Product Data
Clone Name	2A10 (2A10/11)
Applications	ELISA, FC, IF
Host	Mouse
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.
Specificity	This antibody recognizes and binds porcine CD163. This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID: 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID:23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID:21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID: 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID: 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Pérez et al, 2008).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Database Link	UniProt ID Q2VL90
Synonyms	M130; MM130
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.