H3 Rabbit Monoclonal Antibody [Clone ID: BT164]

Specifications

Product Data
Clone Name	BT164
Applications	ELISA, ICC, IF, IHC, IP, WB
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	BT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin.
Specificity	The epitope of BT164 was mapped in the N-terminal tail of histone H3 (27-KSAPAT-32) and included the apoptosis-induced trimethylation of K27. BT164 recognizes an apoptosis-induced trimethylation of K27 on N-terminal tail of histone H3 which is a target for autoantibodies in patients and mice with SLE (systemic lupus erythematosus) (van Bavel et al., 2011; pmid: 20699234). Consequently, this antibody is recommended for the detection and studying of SLE. ELISA studies demonstrated that BT164 exhibits preferential activity with the apoptotic nucleosomes in comparison with the control nucleosomes (van Bavel et al., 2011; pmid: 20699234). It can be used for the detection of apoptosis in general. Furthermore, this antibody was demonstrated to label cells with methylated histone-3 on K27 via immunofluorescence and it also immunoprecipitated chromatin with such a methylation profile, further demonstrating its ability to specifically recognize markers characteristic of SLE (van Bavel et al., 2011; pmid: 20699234).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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