Cytokeratin 7 (KRT7) Rabbit Monoclonal Antibody [Clone ID: OV-TL 12/30]

Specifications

Product Data
Clone Name	OV-TL 12/30
Applications	FC, IF, IHC, WB
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.
Specificity	This antibody recognizes cytokeratin-7. Cytokeratins are a subfamily of intermediate filament proteins but this antibody does not recognize other intermediate filament proteins. The antibody reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry. Cytokeratin 7 (CK7) labeling can help distinguish between lung, breast carcinomas, and urothelial carcinomas that typically stain positive, and colon and prostate carcinomas that typically lack CK7 expression (Jerome et al., 2004; Logani et al., 2003; Murray et al., 2004; Roy et al., 2011). CK7 is a common marker of primary lung adenocarcinomas (almost all cases) with a lower specificity since it is also observed in other primary lung carcinomas and non-pulmonary carcinomas (Jerome et al., 2004). Anti-cytokeratin 7 has also been useful in the differential diagnosis of ovarian neoplasms (McCluggage et al., 2005). Furthermore, this antibody is a marker for distinguishing between the conjunctival and corneal surface epithelia which may be used in the diagnosis of limbal stem cell deficiency (LSCD) (Jirsova et al., 2011; pmid: 21693612). Finally, OV-TL 12/30 has proven very effective and popular in immunohistochemistry; for instance, one group used it to label cytokeratin-7 in human term placental samples (König et al., 2014; pmid: 24326460).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	keratin 7
Database Link	Entrez Gene 3855 Human UniProt ID P08729
Synonyms	CK-7; CK7; Cytokeratin-7; K2C7; K7; Keratin-7; MGC3625; MGC129731; sarcolectin; SCL
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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