DHLAG (CD74) Rabbit Monoclonal Antibody [Clone ID: LN-2]

Specifications

Product Data
Clone Name	LN-2
Applications	ELISA, FC, IF, IHC, IP, WB
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunizing mice with SU-DHL-4 lymphoma cells.
Specificity	LN-2 antibody recognizes CD74. It reacts with an epitope residing within 60 amino acids of the extracytoplasmic, COOH terminus of the protein. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). This antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells. Furthermore, this antibody was shown to be capable of distinguishing small cell lung cancer from a non small cell lung cancer as a part of a panel of antibodies (Ioachim et al., 1996). Furthermore, LN2 was successfully used in immunohistochemical and flow cytometric analysis of lung cancer tissue and cells (Ioachim et al., 1996). Finally, this antibody is also useful in immunofluorescence analysis of CD74, as it was very effective in labelling this protein in CD74-transfected COS-7 cells (Leng et al., 2003).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	CD74 molecule
Database Link	Entrez Gene 972 Human UniProt ID P04233
Synonyms	DHLAG; HLA-DR-gamma; HLADG; Ia-GAMMA; Ii; p33
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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