TLR2 Rabbit Monoclonal Antibody [Clone ID: TL2.1]

Specifications

Product Data
Clone Name	TL2.1
Applications	Bl, FC, IF, IP, WB
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunising BALB/c mice with CHO cells transfected with human TLR2 cDNA.
Specificity	This antibody is specific to TLR2 (extracellular domain) and does not crossreact with human TLR1 or TLR4. TLR2 is involved in the immunological response against bacterial lipoproteins and other microbial cell wall components. TL2.1 antibody has been reported as an effective inhibitor of TLR2-dependent cytokine production and might be used to control and manipulate signalling pathways related to TLR2 (Flo et al., 2000). This antibody has also been exstensively used in flow cytometry and TL2.1 is recommended for the use in flow cytometric investigation of TLR2 expression (Jamin et al., 2015; Wang et al., 2016). For instance, one group treated dendritic cells with azithromycin and observed that this substance supressed TLR2 expression detected via flow cytometry (Lin et al., 2016). Finally, this antibody has been successfully applied in immunofluorescence analysis of TLR2 expression in various cells. One group utilised TL2.1 antibody to investigate TLR2 expression (among others pathogen-related receptors) on SRIK-NKL cells. CD8+ NK/T cell line (Srivastava and Srivastava, 2005).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	toll like receptor 2
Database Link	Entrez Gene 7097 Human UniProt ID O60603
Synonyms	CD282; TIL4
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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