Tnfsf12 Rabbit Monoclonal Antibody [Clone ID: MTW-1]

Specifications

Product Data
Clone Name	MTW-1
Applications	Bl, FC
Reactivity	Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunising SD rats with CD8-mTWEAK, a fusion of the human CD8alpha extracellular region to murine TWEAK.
Specificity	This antibody is specific for murine TWEAK. It has been shown not to react with TNF-α, FasL, and TRAIL. The specificity of this antibody has been confirmed in flow cytometric analysis, where it reacts specifically with mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells transfected with related TNF family members (Nakayama et al, 2003). Additionally, this antibody has been used to assess TWEAK cell surface expression in flow cytometric analysis (Nakayama et al, 2003). This antibody has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When administered to collagen-induced arthritis (CIA) mice, this blocking antibody has been shown to have anti-inflammatory and antiangiogenic effects, with associated improvements to both the clinical and histological manifestations of CIA (Kamata et al, 2006). Additionally, administration of this antibody to denervated mice attenuates denervation-induced skeletal muscle loss (Mittal et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting from CD163 deficiency (Akahori et al, 2015).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	tumor necrosis factor (ligand) superfamily, member 12
Database Link	Entrez Gene 21944 Mouse UniProt ID O54907
Synonyms	APO3L; DR3LG; MGC20669; MGC129581; TWEAK
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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