Ly6g Rat Monoclonal Antibody [Clone ID: RB6-8C5]

Specifications

Product Data
Clone Name	RB6-8C5
Applications	FC, IHC, WB
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
Specificity	This antibody is specific for murine Ly6G, a a 21-25 kDa glycosylphosphatidylinositol (GPI)-linked protein expressed by the myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express Ly6G transiently during bone marrow development, while Ly6G expression in granulocytes and peripheral neutrophils directly correlates with the cell’s level of differentiation and maturation. This hallmark makes Ly6G a good marker for these particular cell populations. Ly6G has also been implicated in the development of antitumor responses. This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to elucidate the origin and roles of macrophages in the post-myocardial infarction remodelling (Sager et al, 2016), to investigate the pathogenesis of primary biliary cirrhosis (Yang et al, 2016), to confirm the phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice (Ricard et al, 2016), and to highlight an important ageing-related defect in the mucosal immune system's ability to sample lumenal antigens (Kobayashi et al, 2013). This antibody has also been used in vivo, for example, to deplete Treg cells when delineating the paralleled developments of inflammation and immunosuppression in tumor microenvironment (Yang et al, 2010), or to deplete neutrophils when underpinning a causal role for neutrophils in severe asthma (Wilson et al, 2009).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	lymphocyte antigen 6 complex, locus G
Database Link	Entrez Gene 546644 Mouse UniProt ID P35461
Synonyms	Ly6g
Reference Data

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